Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.
Identifieur interne : 004113 ( Main/Exploration ); précédent : 004112; suivant : 004114Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.
Auteurs : Tine V. Karlsen [Norvège] ; Emmet Mccormack ; Maja Mujic ; Olav Tenstad ; Helge WiigSource :
- American journal of physiology. Heart and circulatory physiology [ 1522-1539 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Albumines.
- physiologie : Lymphe, Système lymphatique.
- Animaux, Colorants fluorescents, Femelle, Radiopharmaceutiques, Rats, Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Albumins.
- physiology : Lymph, Lymphatic System.
- Animals, Female, Fluorescent Dyes, Mice, Radiopharmaceuticals, Rats.
Abstract
There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and subsequent calculation of removal rate constants (k). BSA was conjugated with Alexa680 NHS ester and remained stable in protein-rich solutions without free dye dissociation. To assess lymph flow, mice or rats were imaged every 30 or 60 min during a 3- to 6-h period following an intradermal injection of 0.5 or 1 μl Alexa680-albumin. Mice were awake between measurements, whereas rats were anesthetized throughout the experiment. The k, a parameter defined as equivalent to lymph flow, was calculated from the slopes of the resultant log-linear washout curves and averaged -0.40 ± 0.03 and -0.30 ± 0.02%/min for control C57BL/6 and C3H mice, respectively. Local administration of the vasoconstrictor endothelin-1 in mice led to a significant reduction in k, whereas overhydration in rats increased k, reflecting the coupling between capillary filtration and lymph flow. Furthermore, k was 50% of wild type in lymphedema Chy mice where dermal lymphatics are absent. We conclude that lymph flow can be determined as its rate constant k by optical imaging of depot clearance of submicroliter amounts of Alexa680-albumin. The method offers a minimally invasive, reproducible, and simple alternative to assess lymphatic function in mice and rats.
DOI: 10.1152/ajpheart.00842.2011
PubMed: 22101523
Affiliations:
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Karlsen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen, Norway. tine.karlsen@biomed.uib.no</nlm:affiliation><country xml:lang="fr">Norvège</country><wicri:regionArea>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen</wicri:regionArea><wicri:noRegion>Bergen</wicri:noRegion></affiliation></author><author><name sortKey="Mccormack, Emmet" sort="Mccormack, Emmet" uniqKey="Mccormack E" first="Emmet" last="Mccormack">Emmet Mccormack</name></author><author><name sortKey="Mujic, Maja" sort="Mujic, Maja" uniqKey="Mujic M" first="Maja" last="Mujic">Maja Mujic</name></author><author><name sortKey="Tenstad, Olav" sort="Tenstad, Olav" uniqKey="Tenstad O" first="Olav" last="Tenstad">Olav Tenstad</name></author><author><name sortKey="Wiig, Helge" sort="Wiig, Helge" uniqKey="Wiig H" first="Helge" last="Wiig">Helge Wiig</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22101523</idno><idno type="pmid">22101523</idno><idno type="doi">10.1152/ajpheart.00842.2011</idno><idno type="wicri:Area/PubMed/Corpus">002377</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002377</idno><idno type="wicri:Area/PubMed/Curation">002377</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002377</idno><idno type="wicri:Area/PubMed/Checkpoint">002377</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002377</idno><idno type="wicri:Area/Ncbi/Merge">004847</idno><idno type="wicri:Area/Ncbi/Curation">004847</idno><idno type="wicri:Area/Ncbi/Checkpoint">004847</idno><idno type="wicri:Area/Main/Merge">004127</idno><idno type="wicri:Area/Main/Curation">004113</idno><idno type="wicri:Area/Main/Exploration">004113</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.</title><author><name sortKey="Karlsen, Tine V" sort="Karlsen, Tine V" uniqKey="Karlsen T" first="Tine V" last="Karlsen">Tine V. Karlsen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen, Norway. tine.karlsen@biomed.uib.no</nlm:affiliation><country xml:lang="fr">Norvège</country><wicri:regionArea>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen</wicri:regionArea><wicri:noRegion>Bergen</wicri:noRegion></affiliation></author><author><name sortKey="Mccormack, Emmet" sort="Mccormack, Emmet" uniqKey="Mccormack E" first="Emmet" last="Mccormack">Emmet Mccormack</name></author><author><name sortKey="Mujic, Maja" sort="Mujic, Maja" uniqKey="Mujic M" first="Maja" last="Mujic">Maja Mujic</name></author><author><name sortKey="Tenstad, Olav" sort="Tenstad, Olav" uniqKey="Tenstad O" first="Olav" last="Tenstad">Olav Tenstad</name></author><author><name sortKey="Wiig, Helge" sort="Wiig, Helge" uniqKey="Wiig H" first="Helge" last="Wiig">Helge Wiig</name></author></analytic><series><title level="j">American journal of physiology. Heart and circulatory physiology</title><idno type="eISSN">1522-1539</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Albumins (metabolism)</term><term>Animals</term><term>Female</term><term>Fluorescent Dyes</term><term>Lymph (physiology)</term><term>Lymphatic System (physiology)</term><term>Mice</term><term>Radiopharmaceuticals</term><term>Rats</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Albumines (métabolisme)</term><term>Animaux</term><term>Colorants fluorescents</term><term>Femelle</term><term>Lymphe (physiologie)</term><term>Radiopharmaceutiques</term><term>Rats</term><term>Souris</term><term>Système lymphatique (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Albumins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Albumines</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Lymphe</term><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lymph</term><term>Lymphatic System</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Fluorescent Dyes</term><term>Mice</term><term>Radiopharmaceuticals</term><term>Rats</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Colorants fluorescents</term><term>Femelle</term><term>Radiopharmaceutiques</term><term>Rats</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and subsequent calculation of removal rate constants (k). BSA was conjugated with Alexa680 NHS ester and remained stable in protein-rich solutions without free dye dissociation. To assess lymph flow, mice or rats were imaged every 30 or 60 min during a 3- to 6-h period following an intradermal injection of 0.5 or 1 μl Alexa680-albumin. Mice were awake between measurements, whereas rats were anesthetized throughout the experiment. The k, a parameter defined as equivalent to lymph flow, was calculated from the slopes of the resultant log-linear washout curves and averaged -0.40 ± 0.03 and -0.30 ± 0.02%/min for control C57BL/6 and C3H mice, respectively. Local administration of the vasoconstrictor endothelin-1 in mice led to a significant reduction in k, whereas overhydration in rats increased k, reflecting the coupling between capillary filtration and lymph flow. Furthermore, k was 50% of wild type in lymphedema Chy mice where dermal lymphatics are absent. We conclude that lymph flow can be determined as its rate constant k by optical imaging of depot clearance of submicroliter amounts of Alexa680-albumin. The method offers a minimally invasive, reproducible, and simple alternative to assess lymphatic function in mice and rats.</div></front></TEI><affiliations><list><country><li>Norvège</li></country></list><tree><noCountry><name sortKey="Mccormack, Emmet" sort="Mccormack, Emmet" uniqKey="Mccormack E" first="Emmet" last="Mccormack">Emmet Mccormack</name><name sortKey="Mujic, Maja" sort="Mujic, Maja" uniqKey="Mujic M" first="Maja" last="Mujic">Maja Mujic</name><name sortKey="Tenstad, Olav" sort="Tenstad, Olav" uniqKey="Tenstad O" first="Olav" last="Tenstad">Olav Tenstad</name><name sortKey="Wiig, Helge" sort="Wiig, Helge" uniqKey="Wiig H" first="Helge" last="Wiig">Helge Wiig</name></noCountry><country name="Norvège"><noRegion><name sortKey="Karlsen, Tine V" sort="Karlsen, Tine V" uniqKey="Karlsen T" first="Tine V" last="Karlsen">Tine V. Karlsen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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